Recombinant enhanced green fluorescent protein (EGFP) (Gene Bank Accession no. E. coli(1) E.coli(2) Yeast(1) By Tag. U57607) is a protein composed of 293 amino acid residues (32.7 kDa) that has an isoelectric point of 6.2 and exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Enhanced green fluorescent protein (EGFP) refers to a recombinant fluorescent protein that is widely used for transfection studies. Order product ABIN412983.
Here we transduced human MSCs with enhanced green fluorescent protein (EGFP). The green fluorescent protein (GFP) from the jellyfishAequorea victoriahas become an important marker of gene expression.However, the sensitivity of wild-type GFP has been below that of standard reporter proteins, such as β-galactosidase, which utilize enzymatic amplification. It is often used to determine the efficiency of gene transfer. Immunization with this peptide stimulates IFNg production, thus making this peptide a model … La protéine fluorescente verte (souvent abrégé GFP, de l'anglais « Green Fluorescent Protein ») est une protéine ayant la propriété d'émettre une fluorescence de couleur verte.Issue d'une méduse (Aequorea victoria), cette protéine est intrinsèquement fluorescente sous l'action d'une enzyme, l'aequoréine, une luciférase qui agit en présence de calcium .
Even with modifications, the enhanced versions in this class of fluorescent protein (EBFP and ECFP) are only about 25 to 40 percent as bright as enhanced green fluorescent protein. Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo.GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. The green fluorescent protein (GFP) and its variants are used in many studies to determine the subcellular localization of other proteins by analyzing fusion proteins. Enhanced Green Fluorescent Protein (EGFP) is one of the most widely used engineered variants of the original wild-type Green Fluorescent Protein.
Enhanced Green Fluorescent Protein, EGFP (200 - 208) HYLSTQSAL: Size: 1 mg: Catalog # AS-65302: US$ $77: Purity % Peak Area By HPLC ≥ 95%: Description: This peptide is H2-Kd-restricted enhanced green fluorescent protein (EGFP)-derived peptide (200-208) and represents a CD8 T cell epitope. For price inquiries, please feel free to contact us through the form below. Enhanced Green Fluorescent Protein (EGFP) EGFP Related Products .
Enhanced GFP Lentivirus (Puromycin) Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding.
The protein can be localized in transfected cells without the need for staining or addition of co-factors. Species: Aequorea victoria.
Therefore, this study attempted to use a recombinant protein. Enhanced Green Fluorescent Protein (EGFP) Chromophore Formation The GFP chromophore is encoded by the primary amino acid sequence, and forms spontaneously without the requirement for cofactors or external enzyme components (other than molecular oxygen), through a self-catalyzed protein folding mechanism and intramolecular rearrangement. Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase brightness and folding efficiency. Recombinant Enhanced Green Fluorescent Protein (eGFP) Protein. We further compared the performance of stagRFP in green-red FRET-biosensing with the recently developed ultra-bright red fluorescent protein mScarlet 11 … We will get back to you as soon as possible.
Welcome! The main problem for nuclear localization studies is the fact that, to some extent, GFP translocates to the nucleus on its own. Aequorea victoria(1) E. coli(1) Human Cytomegalovirus(1) By Source. His(3) His|Myc(1) Price Inquiry.
By Species. Enhanced green fluorescent protein (EGFP) is a novel marker gene product, which is readily detectable using techniques of fluorescence microscopy, flow cytometry, or macroscopic imaging.
We compared the capacity of control and EGFP-positive cells to proliferate under normal culture conditions, as well as express neural markers following trans-differentiation.